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11211 Effects on Other Maillard Reaction Products In all proposals the aim is to reduce the amounts of AA Since AA is formed by the Maillard reaction, one can suspect that the amounts of other Maillard reaction products (MRPs) are in uenced as well, unknown if the concentrations increase or decrease This has caused some concern, for two reasons First, is it possible that the concentrations of other MRPs, as toxic as AA, increase and thereby create a bigger problem than initially Whether this is the case or not is not known There are, however, some indications of possible MRPs to investigate further Chaudhry et al (9) compiled all MRPs, as part of the HEATOX project, known to be present in food and performed molecular modeling to predict mutagenicity and carcinogenicity.

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The database contains 740 compounds that may be formed in the Maillard reaction or through lipid oxidation The probabilities of being mutagenic and/or carcinogenic were calculated using a toxicity expert system Fifty-two compounds were predicted to be both genotoxic and carcinogenic (see Tables 111 and 112) All lipid oxidation products have been found in cooked foods whereas six of the MRPs have not yet been identi ed Unfortunately, there are no systematic investigations on the concentrations of these suspected mutagens/carcinogens in cooked foods where the amounts of AA have been decreased after applying one or more of the mitigation strategies mentioned A way forward could be to test the compounds in Tables 111 and 112 for genotoxicity and then develop a multi-method for detection of the compounds that turned out to be genotoxic.

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Factoring the DCT Matrix In 6 we explained that two matrices are multiplied by taking the dot product of the rows of the first matrix with the columns of the second matrix When we use a matrix to calculate the IDCT we use Equation 101, where Mis the transform matrix and T is the input data to the transform Equation 101 Inverse DCT V = MTTM Since matrix multiplication is associative, we can perform the two multiplication operations in either order For consistency, in this chapter we are going to perform the multiplication as V = MT(TM) In other words, we are

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The effects of different mitigation strategies directed toward AA could then be studied to ensure that concentrations of mutagenic MRPs do not increase 11212 Effects on Hydroxymethylfurfural From the data available it seems especially interesting to study 5-(hydroxymethyl)-2-furancarboxaldehyde, trivial name hydroxymethylfurfural (HMF), present in high concentrations in normal Western diets HMF has not been tested so far for carcinogenicity in experimental animals In addition, there are no epidemiological data available The genotoxic activity is rather weak in a number of test systems, but this might be due to low activity of sulfotransferases necessary to activate HMF to its sulfonated DNA-reactive.

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TABLE 11.1 Lipid oxidation products suspected to be mutagenic and carcinogenic.a 3-penten-2-one [E]-2-butenal [E]-2-nonenal trans-4,5-epoxy-[E]-2-decenal [E]-2-butenoic acid 4-hydroxydecanoic acid lactone 4-hydroxy-2-decenoic acid lactone

1-octen-3-one [Z]-2-butenal [Z]-2-nonenal [E,E]-2,4-undecadienal 4-hydroxynonanoic acid lactone 5-hydroxydecanoic acid lactone 4-hydroxy-2-nonenoic acid lactone

Compiled from Chaudhry et al. (9).

7 1 1 5 6 1 5 5

A zero escape code followed by a value greater than two gives a count of literal bytes to copy to the image The literal bytes immediately follow the count If the count is odd, one fill byte is stored after the data bytes This code is used to store uncompressed data It is worth noting from the preceding description that if the image contains no consecutive bytes with the same value, the resulting image data will be larger than the equivalent uncompressed data RLE4 The RLE4 format is almost identical to the RLE8 format The major difference is that when encoding color runs, the data byte that follows the count contains two pixel values The value in the four high-order bits is used to encode the first and all subsequent odd pixels in the run, while the four low-order bits are used to encode the second and remaining even pixels Thus, the RLE4 format can encode runs of the same pixel value or two alternating values This encoded pair

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